Institutional Repository
The UDSpace Institutional Repository collects and disseminates research material from the University of Delaware.
- Faculty, staff, and graduate students can deposit their research material directly into UDSpace. Faculty may use UDSpace to fulfill the University of Delaware Faculty Senate Open Access Resolution, and in many cases may use it to fulfill open access requirements from grant funding agencies.
- Departments can use UDSpace to publish or distribute their working papers, technical reports, or other research material.
- UDSpace also includes all doctoral dissertations from winter 2014 forward, and all master's theses from fall 2009 forward.
To learn more about UDSpace, and how you can make your research openly accessible to the public, visit our UDSpace Policies website.
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Recent Submissions
Spatial transcriptomics reveals alterations in perivascular macrophage lipid metabolism in the onset of Wooden Breast myopathy in broiler chickens
(Scientific Reports, 2024-02-11) Wang, Ziqing; Khondowe, Paul; Brannick, Erin; Abasht, Behnam
This study aims to use spatial transcriptomics to characterize the cell-type-specific expression profile associated with the microscopic features observed in Wooden Breast myopathy. 1 cm3 muscle sample was dissected from the cranial part of the right pectoralis major muscle from three randomly sampled broiler chickens at 23 days post-hatch and processed with Visium Spatial Gene Expression kits (10X Genomics), followed by high-resolution imaging and sequencing on the Illumina Nextseq 2000 system. WB classification was based on histopathologic features identified. Sequence reads were aligned to the chicken reference genome (Galgal6) and mapped to histological images. Unsupervised K-means clustering and Seurat integrative analysis differentiated histologic features and their specific gene expression pattern, including lipid laden macrophages (LLM), unaffected myofibers, myositis and vasculature. In particular, LLM exhibited reprogramming of lipid metabolism with up-regulated lipid transporters and genes in peroxisome proliferator-activated receptors pathway, possibly through P. Moreover, overexpression of fatty acid binding protein 5 could enhance fatty acid uptake in adjacent veins. In myositis regions, increased expression of cathepsins may play a role in muscle homeostasis and repair by mediating lysosomal activity and apoptosis. A better knowledge of different cell-type interactions at early stages of WB is essential in developing a comprehensive understanding.
Post-harvest drone flights to measure weed growth and yield associations
(Agricultural & Environmental Letters, 2022-06-14) Miller, Jarrod O.; Shober, Amy L.; VanGessel, Mark J.
Drone flights are often only performed during the growing season, with no data collected once harvest has been completed, although they could be used to measure winter annual weed growth. Using a drone mounted with a multispectral sensor, we flew small plot corn (Zea mays L.) fertility, cover crop, and population studies at black layer and 0–14 d after harvest (DAH). Yields had positive correlations to normalized difference vegetation index (NDVI) at black layer but often had negative correlations to corn yields 0–14 DAH. After harvest, NDVI could be associated with weed growth, and negative correlations to yield could point to reduced corn canopy allowing light to reach late-season weeds. In fertility studies, excess nitrogen appears to increase weed biomass after harvest, which can be easily identified through drone imagery. Flights should be performed after corn harvest as weed growth may provide additional insight into management decisions.
Core Ideas:
- Corn yields can be correlated to post-harvest weed biomass by using NDVI.
- Drone flights efficiently mapped weeds and made correlations to yield and management.
- Fall weed control can be prioritized using drone mapping.
Abbreviations:
DAH
days after harvest
LAI
leaf area index
NDVI
normalized difference vegetation index
Monitoring winter wheat growth at different heights using aerial imagery
(Agronomy Journal, 2021-02-09) Miller, Jarrod O.; Adkins, James
Drones (unmanned aerial vehicles) provide another system to mount sensors for measuring plant characteristics. For winter wheat (Triticum aestivum) this can include evaluating stands and making nitrogen (N) recommendations. Timing these flights and adequate camera resolution (based on flying height), must be known before applying tasks. This study observed six winter wheat planting populations (222, 297, 371, 445, 494, and 544 seeds m–2) at three different heights above ground level (30, 60, and 120 m) over three growing seasons. Plant populations could be separated at all growth stages and heights flown but were easier to separate right after emergence (GS11). In the spring, additional tillering caused the higher populations (371–544 seeds m–2) to have similar normalized difference vegetative index (NDVI), much like the final yields. Comparing changes in NDVI between flights was also successful in separating high and low planting populations, with inverse relationships in the fall and spring. A random forest classification of tiller counts by NDVI measurements ranked change in NDVI between stages as the most important compared to single flights. As separation and classification was successful at the lowest camera resolution (120 m), it can be possible for scouts to collect whole field imagery for analyses prior to deciding on split N applications.
Sensor-based measurements of NDVI in small grain and corn fields by tractor, drone, and satellite platforms
(Crop and Environment, 2024-02-01) Miller, Jarrod O.; Mondal, Pinki; Sarupria, Manan
The use of sensors for variable rate nitrogen (VRN) applications is transitioning from equipment-based to drone and satellite technologies. However, regional algorithms, initially designed for proximal active sensors, require evaluation for compatibility with remotely sensed reflectance and N-rate predictions. This study observed normalized difference vegetation index (NDVI) data from six small grain and two corn fields over three years. We employed three platforms: tractor-mounted active sensors (T-NDVI), passive multispectral drone (D-NDVI), and satellite (S-NDVI) sensors. Averaged NDVI values were extracted from the as-applied equipment polygons. Correlations between NDVI values from the three platforms were positive and strong, with D-NDVI consistently recording the highest values, particularly in areas with lower plant biomass. This was attributed to D-NDVI's lower soil reflectance and its ability to measure the entire biomass within equipment polygons. For small grains, sensors spaced on equipment booms might not capture accurate biomass in poor-growing and low NDVI regions. Regarding VRN, S-NDVI and D-NDVI occasionally aligned with T-NDVI recommendations but often suggested half the active sensor rate. Final yields showed some correlation with landscape variables, irrespective of N application. This finding suggests the potential use of drone or satellite imagery to provide multiple NDVI maps before application, incorporating expected landscape responses and thereby enhancing VRN effectiveness.
Core–Shell Rubber Nanoparticle-Modified CFRP/Steel Ambient-Cured Adhesive Joints: Curing Kinetics and Mechanical Behavior
(Materials, 2024-02-04) Okeola, Abass Abayomi; Hernandez-Limon, Jorge E.; Tatar, Jovan
Externally bonded wet-layup carbon fiber-reinforced polymer (CFRP) strengthening systems are extensively used in concrete structures but have not found widespread use in deficient steel structures. To address the challenges of the adhesive bonding of wet-layup CFRP to steel substrates, this study investigated the effect of core–shell rubber (CSR) nanoparticles on the curing kinetics, glass transition temperature (𝑇𝑔) and mechanical properties of ambient-cured epoxy/CSR blends. The effects of silane coupling agent and CSR on the adhesive bond properties of CFRP/steel joints were also investigated. The results indicate that CSR nanoparticles have a mild catalytic effect on the curing kinetics of epoxy under ambient conditions. The effect of CSR on the 𝑇𝑔 of epoxy was negligible. Epoxy adhesives modified with 5 to 20%wt. of CSR nanoparticles were characterized with improved ductility over brittle neat epoxy; however, the addition of CSR nanoparticles reduced tensile strength and modulus of the adhesives. An up to 250% increase in the single-lap shear strength of CFRP/steel joints was accomplished in CSR-modified joints over neat epoxy adhesive joints.