D1FHS, the Type Strain of the Ammonia-Oxidizing Bacterium Nitrosococcus wardiae spec. nov.: Enrichment, Isolation, Phylogenetic, and Growth Physiological Characterization
Date
4/14/16
Journal Title
Journal ISSN
Volume Title
Publisher
Frontiers Media S.A.
Abstract
An ammonia-oxidizing bacterium, strain D1FHS, was enriched into pure culture from a sediment sample retrieved in Jiaozhou Bay, a hyper-eutrophic semi-closed water body hosting the metropolitan area of Qingdao, China. Based on initial 16S rRNA gene sequence analysis, strain D1FHS was classified in the genus Nitrosococcus, family Chromatiaceae, order Chromatiales, class Gammaproteobacteria; the 16S rRNA gene sequence with highest level of identity to that of D1FHS was obtained from Nitrosococcus halophilus Nc4T. The average nucleotide identity between the genomes of strain D1FHS and N. halophilus strain Nc4 is 89.5%. Known species in the genus Nitrosococcus are obligate aerobic chemolithotrophic ammonia-oxidizing bacteria adapted to and restricted to marine environments. The optimum growth (maximum nitrite production) conditions for D1FHS in a minimal salts medium are: 50 mM ammonium and 700 mM NaCl at pH of 7.5 to 8.0 and at 37 degrees C in dark. Because pertinent conditions for other studied Nitrosococcus spp. are 100200 mM ammonium and <700 mM NaCl at pH of 7.5 to 8.0 and at 2832 degrees C, D1FHS is physiologically distinct from other Nitrosococcus spp. in terms of substrate, salt, and thermal tolerance.
Description
Publisher's PDF
Keywords
Citation
Wang, L., Lim, C. K., Dang, H., Hanson, T. E., & Klotz, M. G. (2016). D1FHS, the type strain of the ammonia-oxidizing bacterium nitrosococcus wardiae spec. nov.: Enrichment, isolation, phylogenetic, and growth physiological characterization. Frontiers in Microbiology, 7, 512. doi:10.3389/fmicb.2016.00512