The creation of reagents fibulin-1C/D and truncates for protein purification and apoptotic ability on PCa cell lines
Date
2018
Authors
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Publisher
University of Delaware
Abstract
Prostate Cancer (PCa) is the most diagnosed type of cancer in men in the United States. The 5 year survival rate in men for nonmetastatic cancer is around 100%, but when the cancer turns metastatic the 5 year survival rate drops to 30%. The issue for doctors is that it is difficult to tell whether a cancer will turn metastatic and if antibody therapy and excision of the tumor are not enough then patients do not have a successful options for therapy. Sikes’ lab studied the bone microenvironment and how bone cells interact with PCa cells to aid in PCa invasion and proliferation. It was found that HS-5 cells secret a factor that induces apoptosis in PCa cells. That factor was found to be fibulin-1. At metastatic sites, cancers recruit other neighboring cells to aid in the PCa invasion and proliferation due to the factors the PCa secretes or upregulates in other cell types. One of those factors highly secreted in tumor supportive cells is TGF beta, once HS-5 cells are incubated in TGF beta they lose their ability to induce apoptosis to PCa, as well as the expression of fibulin-1. This has led to the supposition that the bone is originally a hostile place for PCa metastasis until HS-5 is remodeled. This being said, fibulin-1 has potential to be utilized as a marker in serum of PCa patients to determine the relative risk of the prostate cancer spreading and possibly a treatment.