Browsing by Author "Pitel, F."
Now showing 1 - 4 of 4
Results Per Page
Sort Options
Item Fatness QTL on chicken chromosome 5 and interaction with sex(EDP Sciences, 2006) Abasht, Behnam; Pitel, F.; Lagarrigue, S.; Le Bihan-Duval, Elisabeth; Le Roy, P.; Demeure, O.; Vignoles, F.; Simon, Jean; Cogburn, Larry A.; Aggrey, S. E. (Samuel E.); Vignal, A.; Douaire, M.; Abasht, B., Pitel, F., Lagarrigue, S., Le Bihan-Duval, E., Le Roy, P., Demeure, O., Vignoles, F., Simon, J., Cogburn, L., Aggrey, S., Vignal, A., Douaire, M.; Abasht, Behnam; Cogburn, Larry A.Quantitative trait loci (QTL) affecting fatness in male chickens were previously identified on chromosome 5 (GGA5) in a three-generation design derived from two experimental chicken lines divergently selected for abdominal fat weight. A new design, established from the same pure lines, produced 407 F2 progenies (males and females) from 4 F1-sire families. Body weight and abdominal fat were measured on the F2 at 9 wk of age. In each sire family, selective genotyping was carried out for 48 extreme individuals for abdominal fat using seven microsatellite markers from GGA5. QTL analyses confirmed the presence of QTL for fatness on GGA5 and identified a QTL by sex interaction. By crossing one F1 sire heterozygous at the QTL with lean line dams, three recombinant backcross 1 (BC1) males were produced and their QTL genotypes were assessed in backcross 2 (BC2) progenies. These results confirmed the QTL by sex interaction identified in the F2 generation and they allow mapping of the female QTL to less than 8 Mb at the distal part of the GGA5. They also indicate that fat QTL alleles were segregating in both fat and lean lines.Item A high-resolution radiation hybrid map of chicken chromosome 5 and comparison with human chromosomes(BioMed Central, 2004) Pitel, F.; Abasht, Behnam; Morisson, M.; Crooijmans, R. P.; Vignoles, F.; Leroux, S.; Feve, K.; Bardes, S.; Milan, D.; Lagarrigue, S.; Groenen, M. A.; Douaire, M.; Vignal, A.; Pitel, F., Abasht, B., Morisson, M., Crooijmans, R. P., Vignoles, F., Leroux, S., Feve, K., Bardes, S., Milan, D., Lagarrigue, S., Groenen, M. A., Douaire, M., Vignal, A.; Abasht, BehnamThe resolution of radiation hybrid (RH) maps is intermediate between that of the genetic and BAC (Bacterial Artificial Chromosome) contig maps. Moreover, once framework RH maps of a genome have been constructed, a quick location of markers by simple PCR on the RH panel is possible. The chicken ChickRH6 panel recently produced was used here to construct a high resolution RH map of chicken GGA5. To confirm the validity of the map and to provide valuable comparative mapping information, both markers from the genetic map and a high number of ESTs (Expressed Sequence Tags) were used. Finally, this RH map was used for testing the accuracy of the chicken genome assembly for chromosome 5.Item Mapping quantitative trait loci affecting fatness and breast muscle weight in meat-type chicken lines divergently selected on abdominal fatness(EDP Sciences, 2006) Lagarrigue, S.; Pitel, F.; Carre, W.; Abasht, Behnam; Le Roy, P.; Neau, A.; Amigues, Y.; Sourdioux, M.; Simon, Jean; Cogburn, Larry A.; Aggrey, S. E. (Samuel E.); Leclercq, B.; Vignal, A.; Douaire, M.; Lagarrigue, S., , Pitel, F., Carre, W., Abasht, B., Le Roy, P., Neau, A., Amigues, Y., Sourdioux, M., Simon, J., Cogburn, L., Aggrey, S., Leclercq, B., Vignal, A., Douaire, M.; Carre, W.; Abasht, Behnam; Cogburn, Larry A.Quantitative trait loci (QTL) for abdominal fatness and breast muscle weight were investigated in a three-generation design performed by inter-crossing two experimental meattype chicken lines that were divergently selected on abdominal fatness. A total of 585 F2 male offspring from 5 F1 sires and 38 F1 dams were recorded at 8 weeks of age for live body, abdominal fat and breast muscle weights. One hundred-twenty nine microsatellite markers, evenly located throughout the genome and heterozygous for most of the F1 sires, were used for genotyping the F2 birds. In each sire family, those offspring exhibiting the most extreme values for each trait were genotyped. Multipoint QTL analyses using maximum likelihood methods were performed for abdominal fat and breast muscle weights, which were corrected for the effects of 8-week body weight, dam and hatching group. Isolated markers were assessed by analyses of variance. Two significant QTL were identified on chromosomes 1 and 5 with effects of about one within-family residual standard deviation. One breast muscle QTL was identified on GGA1 with an effect of 2.0 within-family residual standard deviation.Item Using transcriptome profiling to characterize QTL regions on chicken chromosome 5(BioMed Central, 2009) Le Mignon, G.; Pitel, F.; Desert, C.; Leroux, S.; Demeure, O.; Guernec, G.; Abasht, Behnam; Douaire, M.; Le Roy, P.; Lagarrigue, S.; Le Mignon, G., Pitel, F., Desert, C., Leroux, S., Demeure, O., Guernec, G., Abasht, Behnam, Douaire, M., Le Roy, P., Lagarrigue, S.; Abasht, BehnamAlthough many QTL for various traits have been mapped in livestock, location confidence intervals remain wide that makes difficult the identification of causative mutations. The aim of this study was to test the contribution of microarray data to QTL detection in livestock species. Three different but complementary approaches are proposed to improve characterization of a chicken QTL region for abdominal fatness (AF) previously detected on chromosome 5 (GGA5).