Heat-induced ribosome pausing triggers mRNA co-translational decay in Arabidopsis thaliana
| Author(s) | Merret, R´emy | |
| Author(s) | Nagarajan, Vinay K. | |
| Author(s) | Carpentier, Marie-Christine | |
| Author(s) | Park, Sunhee | |
| Author(s) | Favory, Jean-Jacques | |
| Author(s) | Descombin, Julie | |
| Author(s) | Picart, Claire | |
| Author(s) | Charng, Yee-yung | |
| Author(s) | Green, Pamela J. | |
| Author(s) | Deragon, Jean-Marc | |
| Author(s) | Bousquet-Antonelli, C´ ecile | |
| Ordered Author | R´emy Merret, Vinay K. Nagarajan, Marie-Christine Carpentier, Sunhee Park, Jean-Jacques Favory, Julie Descombin, Claire Picart, Yee-yung Charng, Pamela J. Green, Jean-Marc Deragon and C´ ecile Bousquet-Antonelli | |
| UD Author | Nagarajan, Vinay K. | en_US |
| UD Author | Park, Sunhee | en_US |
| UD Author | Green, Pamela J. | en_US |
| Date Accessioned | 2015-12-23T15:10:18Z | |
| Date Available | 2015-12-23T15:10:18Z | |
| Copyright Date | Copyright © The Author(s) 2015. | en_US |
| Publication Date | 2015-04-06 | |
| Description | Publisher's PDF | en_US |
| Abstract | The reprogramming of gene expression in heat stress is a key determinant to organism survival. Gene expression is downregulated through translation initiation inhibition and release of free mRNPs that are rapidly degraded or stored. In mammals, heat also triggers 5 -ribosome pausing preferentially on transcripts coding for HSC/HSP70 chaperone targets, but the impact of such phenomenon on mRNA fate remains unknown. Here, we provide evidence that, in Arabidopsis thaliana, heat provokes 5 -ribosome pausing leading to the XRN4-mediated 5 -directed decay of translating mRNAs. We also show that hindering HSC/HSP70 activity at 20◦C recapitulates heat effects by inducing ribosome pausing and co-translational mRNA turnover. Strikingly, co-translational decay targets encode proteins with high HSC/HSP70 binding scores and hydrophobic N-termini, two characteristics that were previously observed for transcripts most prone to pausing in animals. This work suggests for the first time that stress-induced variation of translation elongation rate is an evolutionarily conserved process leading to the polysomal degradation of thousands of ‘nonaberrant’ mRNAs. | en_US |
| Department | Delaware Biotechnology Institute. | en_US |
| Citation | Merret, Rémy, et al. "Heat-induced ribosome pausing triggers mRNA co-translational decay in Arabidopsis thaliana." Nucleic acids research (2015): gkv234. | en_US |
| DOI | doi:10.1093/nar/gkv234 | en_US |
| ISSN | 0305-1048 ; e- 1362-4962 | en_US |
| URL | http://udspace.udel.edu/handle/19716/17345 | |
| Language | en_US | en_US |
| Publisher | Oxford University Press | en_US |
| dc.rights | CC-BY NC (http://creativecommons.org/licenses/by-nc/4.0/) | en_US |
| dc.source | Nucleic Acids Research | en_US |
| dc.source.uri | http://nar.oxfordjournals.org/ | en_US |
| Title | Heat-induced ribosome pausing triggers mRNA co-translational decay in Arabidopsis thaliana | en_US |
| Type | Article | en_US |
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