Activity of (-) Astrogorgiadiol
| Author(s) | Raciti, David | |
| Date Accessioned | 2012-10-05T19:22:13Z | |
| Date Available | 2012-10-05T19:22:13Z | |
| Publication Date | 2012-05 | |
| Abstract | (-) Astrogorgiadiol, a naturally occurring analog of calcitriol (1,25-dihydroxyvitamin D3) isolated from the Japanese marine sponge Astrogorgia, has demonstrated unique biological functions. In an effort to study these functions, synthetic routes for obtaining (-) astrogorgiadiol were constructed in 2001 by Dr. Taber and Dr. Malcolm. This thesis reports the several efforts made to increase the cost-effectiveness and productivity of synthetic routes leading to (-) astrogorgiadiol. While pursuing more cost-effective and productive means to synthesize (-) astrogorgiadiol, several milligrams of the compound were obtained and used for biological study. The question that was proposed and investigated was, “What effects does (-) astrogorgiadiol elicit on cancer cells?” To begin gathering data to answer this question, several in-vitro assays were conducted on various cell lines. First, the LD50/ED50 for (-) astrogorgiadiol was determined utilizing two bone cancer cell lines. Then utilizing the LD50/ED50 concentration, cells were exposed to (-) astrogorgiadiol and expression studies were pursued on Osteopontin (OPN), a glycoprotein known for its over-expression in various cancers. | en_US |
| Advisor | Douglass F. Taber | |
| Program | Biochemistry | |
| URL | http://udspace.udel.edu/handle/19716/11560 | |
| Language | en_US | en_US |
| Publisher | University of Delaware | |
| Keywords | (-) astrogorgiadiol | en_US |
| Keywords | synthetic production | en_US |
| Keywords | cancel cells | en_US |
| Keywords | LD50/ED50 | en_US |
| Keywords | Osteopontin | en_US |
| Keywords | OPN | en_US |
| Title | Activity of (-) Astrogorgiadiol | en_US |
| Type | Thesis | en_US |