Antecubital venous endothelial ETB receptor protein expression is preserved with aging in men
| Author(s) | Tummala, Saumya | |
| Author(s) | Kuczmarski, Andrew V. | |
| Author(s) | Del Vecchio, Angelica R. | |
| Author(s) | Schwab, Allyson I. | |
| Author(s) | Edwards, David G. | |
| Author(s) | Wenner, Megan M. | |
| Date Accessioned | 2024-02-14T18:21:14Z | |
| Date Available | 2024-02-14T18:21:14Z | |
| Publication Date | 2024-01-01 | |
| Description | This article was originally published in American Journal of Physiology - Heart and Circulatory Physiology. The version of record is available at: https://doi.org/10.1152/ajpheart.00621.2023. Copyright © 2024 the American Physiological Society. This article will be embargoed until 01/01/2025. | |
| Abstract | Changes in endothelial function precede the development of cardiovascular disease (CVD). We have previously shown that age-related declines in endothelial function in women are due in part to a reduction in endothelial cell endothelin-B receptor (ETBR) protein expression. However, it is not known if ETBR protein expression changes with aging in men. The purpose of this study was to test the hypothesis that ETBR protein expression is attenuated in older men (OM) compared with younger men (YM). Primary endothelial cells were harvested from the antecubital vein of 14 OM (60 ± 6 yr; 26 ± 3 kg/m2) and 17 YM (24 ± 5 yr; 24 ± 2 kg/m2). Cells were stained with 4′,6-diamidino-2-phenylindole, vascular endothelial cadherin, and ETBR. Images were quantified using immunocytochemistry. Endothelial function was assessed using brachial artery flow-mediated dilation (FMD). Systolic BP was similar (OM, 123 ± 11 vs. YM, 122 ± 10 mmHg) whereas diastolic BP was higher in OM (OM, 77 ± 7 vs. YM, 70 ± 6 mmHg; P < 0.01). Total testosterone was lower in OM (OM, 6.28 ± 4.21 vs. YM, 9.10 ± 2.68 ng/mL; P = 0.03). As expected, FMD was lower in OM (OM, 3.85 ± 1.51 vs. YM, 6.40 ± 2.68%; P < 0.01). However, ETBR protein expression was similar between OM and YM (OM, 0.39 ± 0.17 vs. YM, 0.42 ± 0.17 AU; P = 0.66). These data suggest that ETBR protein expression is not altered with age in men. These findings contrast with our previous data in women and further support sex differences in the endothelin system. NEW & NOTEWORTHY Our laboratory has previously shown that age-related declines in endothelial function are associated with a reduction in endothelial cell ETBR protein expression in women. However, it is unclear if endothelial cell ETBR protein expression is reduced with aging in men. This study demonstrates that endothelial cell ETBR protein expression is preserved with aging in men, and provides additional evidence for sex differences in the endothelin system. | |
| Sponsor | This research was supported by National Institutes of Health (NIH) Grant R01 HL 146558 (to M.M.W.) and the Center of Biomedical Research Excellence in Cardiovascular Health’s NIH Grant P20 GM113125 (to D.G.E.). DISCLOSURES M.M.W. is a paid consultant for Orchestra Biomed and has received financial compensation. None of the other authors has any conflicts of interest, financial or otherwise, to disclose. | |
| Citation | Tummala, Saumya, Andrew V. Kuczmarski, Angelica R. Del Vecchio, Allyson I. Schwab, David G. Edwards, and Megan M. Wenner. “Antecubital Venous Endothelial ETB Receptor Protein Expression Is Preserved with Aging in Men.” American Journal of Physiology-Heart and Circulatory Physiology 326, no. 1 (January 1, 2024): H110–15. https://doi.org/10.1152/ajpheart.00621.2023. | |
| ISSN | 1522-1539 | |
| URL | https://udspace.udel.edu/handle/19716/33986 | |
| Language | en_US | |
| Publisher | American Journal of Physiology - Heart and Circulatory Physiology | |
| Keywords | aging | |
| Keywords | endothelial function | |
| Keywords | endothelin-1 | |
| Keywords | flow-mediated dilation | |
| Title | Antecubital venous endothelial ETB receptor protein expression is preserved with aging in men | |
| Type | Article |
Files
Original bundle
1 - 1 of 1
No Thumbnail Available
- Name:
- Antecubital Venous Endothelial ETB Receptor Protein Expression is Preserved with Aging in Men.pdf
- Size:
- 1.06 MB
- Format:
- Adobe Portable Document Format
- Description:
- Main article
License bundle
1 - 1 of 1
No Thumbnail Available
- Name:
- license.txt
- Size:
- 2.22 KB
- Format:
- Item-specific license agreed upon to submission
- Description: