Impact of the GTP Binding Protein ARF-6 on the Biogenesis of Multiple Extracellular Vesicle Subpopulations in C. Elegans
| Author(s) | Wagner, Katherine | |
| Date Accessioned | 2022-10-11T14:00:33Z | |
| Date Available | 2022-10-11T14:00:33Z | |
| Publication Date | 2022-05 | |
| Abstract | Extracellular vesicles (EVs) are nano-sized, membrane-bound vesicles that play crucial roles in intercellular communication, impacting both physiologic and pathophysiologic pathways. Use of the genetic model organism C. elegans allows us to study and track EVs and their cargoes in vivo, to observe EV biogenesis and shedding. C. elegans EVs contain various different cargoes, including the calcium homeostasis modulator CLHM-1 and the polycystin PKD-2 ion channels. These cargoes are found to be in two different EV subpopulations that bud from different locations on the cilia of male tail sensory neurons. The small GTPase ARF-6 has been suggested to participate in microvesicle shedding via the phospholipase D pathway of signal transduction based on in vitro studies. However, whether ARF-6 plays this role in the release of EVs from cilia in vivo is unknown. Here, we show, using fluorescent protein tagging and TIRF microscopy, that ARF-6 affects both CLHM-1 and PKD-2-containing EVs, indicating that this protein may act in male-tail sensory neurons to impact biogenesis of all EV subpopulations. Loss of ARF-6 results in an approximate 50% reduction in release of both EV populations, opening the door for several future hypotheses and lines experimentation using the arf-6 mutant. | en_US |
| Advisor | Jessica Tanis | |
| Program | Biology | |
| URL | https://udspace.udel.edu/handle/19716/31468 | |
| Publisher | University of Delaware | en_US |
| Keywords | Extracellular vesicles | en_US |
| Keywords | C. elegans | en_US |
| Keywords | Biogenesis | en_US |
| Title | Impact of the GTP Binding Protein ARF-6 on the Biogenesis of Multiple Extracellular Vesicle Subpopulations in C. Elegans | en_US |
| Type | Thesis | en_US |