Mixotrophy broadens the ecological niche range of the iron oxidizer Sideroxydans sp. CL21 isolated from an iron-rich peatland

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Author(s)Cooper, Rebecca E.
Author(s)Finck, Jessica
Author(s)Chan, Clara
Author(s)Küsel, Kirsten
Date Accessioned2023-04-24T19:59:26Z
Date Available2023-04-24T19:59:26Z
Publication Date2023-02-14
DescriptionThis article was originally published in FEMS Microbiology Ecology. The version of record is available at: https://doi.org/10.1093/femsec/fiac156
AbstractSideroxydans sp. CL21 is a microaerobic, acid-tolerant Fe(II)-oxidizer, isolated from the Schlöppnerbrunnen fen. Since the genome size of Sideroxydans sp. CL21 is 21% larger than that of the neutrophilic Sideroxydans lithotrophicus ES-1, we hypothesized that strain CL21 contains additional metabolic traits to thrive in the fen. The common genomic content of both strains contains homologs of the putative Fe(II) oxidation genes, mtoAB and cyc2. A large part of the accessory genome in strain CL21 contains genes linked to utilization of alternative electron donors, including NiFe uptake hydrogenases, and genes encoding lactate uptake and utilization proteins, motility and biofilm formation, transposable elements, and pH homeostasis mechanisms. Next, we incubated the strain in different combinations of electron donors and characterized the fen microbial communities. Sideroxydans spp. comprised 3.33% and 3.94% of the total relative abundance in the peatland soil and peatland water, respectively. Incubation results indicate Sideroxydans sp. CL21 uses H2 and thiosulfate, while lactate only enhances growth when combined with Fe, H2, or thiosulfate. Rates of H2 utilization were highest in combination with other substrates. Thus, Sideroxydans sp. CL21 is a mixotroph, growing best by simultaneously using substrate combinations, which helps to thrive in dynamic and complex habitats.
SponsorREC, JF, and KK were supported by the Collaborative Research Centre Chemical Mediators in Complex Biosystems (SFB 1127 ChemBioSys, Project Number 239748522), which is funded by the Deutsche Forschungsgemeinschaft. CSC was supported by the National Science Foundation (EAR-1833525).
CitationRebecca E Cooper, Jessica Finck, Clara Chan, Kirsten Küsel, Mixotrophy broadens the ecological niche range of the iron oxidizer Sideroxydans sp. CL21 isolated from an iron-rich peatland, FEMS Microbiology Ecology, Volume 99, Issue 2, February 2023, fiac156, https://doi.org/10.1093/femsec/fiac156
ISSN1574-6941
URLhttps://udspace.udel.edu/handle/19716/32669
Languageen_US
PublisherFEMS Microbiology Ecology
KeywordsSideroxydans sp. CL21
Keywordsgenome
Keywordsphysiology
Keywordspeatland
Keywordsiron oxidation
Keywordsmixotrophy
TitleMixotrophy broadens the ecological niche range of the iron oxidizer Sideroxydans sp. CL21 isolated from an iron-rich peatland
TypeArticle
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