RNA localization to the mitotic spindle is essential for early development and is regulated by kinesin-1 and dynein
| Author(s) | Remsburg, Carolyn M. | |
| Author(s) | Konrad, Kalin D. | |
| Author(s) | Song, Jia L. | |
| Date Accessioned | 2023-04-18T18:08:38Z | |
| Date Available | 2023-04-18T18:08:38Z | |
| Publication Date | 2023-03-06 | |
| Description | This article was originally published in Journal of Cell Science. The version of record is available at: https://doi.org/10.1242/jcs.260528. This article will be embargoed until 3/6/2024. | |
| Abstract | Mitosis is a fundamental and highly regulated process that acts to faithfully segregate chromosomes into two identical daughter cells. Localization of gene transcripts involved in mitosis to the mitotic spindle might be an evolutionarily conserved mechanism to ensure that mitosis occurs in a timely manner. We identified many RNA transcripts that encode proteins involved in mitosis localized at the mitotic spindles in dividing sea urchin embryos and mammalian cells. Disruption of microtubule polymerization, kinesin-1 or dynein results in lack of spindle localization of these transcripts in the sea urchin embryo. Furthermore, results indicate that the cytoplasmic polyadenylation element (CPE) within the 3′UTR of the Aurora B transcript, a recognition sequence for CPEB, is essential for RNA localization to the mitotic spindle in the sea urchin embryo. Blocking this sequence results in arrested development during early cleavage stages, suggesting that RNA localization to the mitotic spindle might be a regulatory mechanism of cell division that is important for early development. | |
| Sponsor | This work was supported by the National Science Foundation (NSF) IOS (1553338) and MCB (2103453) grants to J.L.S., the National Institutes of Health (P20GM103653); a University of Delaware fellowship to C.M.R. and K.D.K., and a Sigma Xi grant to C.M.R. Deposited in PMC for release after 12 months. Microscopy access was supported by grants from the NIH-NIGMS (P20 GM103446), the NIGMS (P20 GM139760) and the State of Delaware. The authors would like to thank Elizabeth McCulla and other undergraduate students in BISC412 who helped generate the RNA in situ probes used in this paper. We also thank Nina Faye Sampilo for the NuMA FISH images. The authors would also like to thank Dr Gary Laverty (University of Delaware) for his kind gift of the LLC-PK1 cells. Figures were created with using Biorender.com. We would also like to thank the anonymous reviewers for their insightful comments. | |
| Citation | Remsburg, Carolyn M., Kalin D. Konrad, and Jia L. Song. “RNA Localization to the Mitotic Spindle Is Essential for Early Development and Is Regulated by Kinesin-1 and Dynein.” Journal of Cell Science 136, no. 5 (March 2023): jcs260528. https://doi.org/10.1242/jcs.260528. | |
| ISSN | 1477-9137 | |
| URL | https://udspace.udel.edu/handle/19716/32657 | |
| Language | en_US | |
| Publisher | Journal of Cell Science | |
| Keywords | Mitosis | |
| Keywords | RNA localization | |
| Keywords | Kinesin-1 | |
| Keywords | Dynein | |
| Keywords | Embryonic development | |
| Title | RNA localization to the mitotic spindle is essential for early development and is regulated by kinesin-1 and dynein | |
| Type | Article |