Transcriptomic Signature of the Simulated Microgravity Response in Caenorhabditis elegans and Comparison to Spaceflight Experiments

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Author(s)Çelen, İrem
Author(s)Jayasinghe, Aroshan
Author(s)Doh, Jung H.
Author(s)Sabanayagam, Chandran R.
Date Accessioned2023-03-27T20:18:25Z
Date Available2023-03-27T20:18:25Z
Publication Date2023-01-10
DescriptionThis article was originally published in Cells . The version of record is available at: https://doi.org/10.3390/cells12020270
AbstractGiven the growing interest in human exploration of space, it is crucial to identify the effects of space conditions on biological processes. Here, we analyze the transcriptomic response of Caenorhabditis elegans to simulated microgravity and observe the maintained transcriptomic response after returning to ground conditions for four, eight, and twelve days. We show that 75% of the simulated microgravity-induced changes on gene expression persist after returning to ground conditions for four days while most of these changes are reverted after twelve days. Our results from integrative RNA-seq and mass spectrometry analyses suggest that simulated microgravity affects longevity-regulating insulin/IGF-1 and sphingolipid signaling pathways. Finally, we identified 118 genes that are commonly differentially expressed in simulated microgravity- and space-exposed worms. Overall, this work provides insight into the effect of microgravity on biological systems during and after exposure.
SponsorThis work was supported by NASA grants NNX12AR59G, NNX10AN63H, and NNX13AM08G, awarded to CRS and Sigma Xi grant G2016100191301836, awarded to İÇ. The BIOMIX computing cluster and the Bioimaging center are supported by Delaware INBRE grant (NIH/NIGMS GM103446) and Delaware EPSCoR grants (NSF EPS-0814251 and NSF IIA-1330446). The University of Delaware Sequencing and Genotyping Center is supported by the NIH NIGMS IDeA Program Grant #P20 GM103446. None of these funding bodies were involved in the design, data collection, analysis, interpretation of data, or in writing the manuscript.
CitationÇelen, İrem, Aroshan Jayasinghe, Jung H. Doh, and Chandran R. Sabanayagam. 2023. "Transcriptomic Signature of the Simulated Microgravity Response in Caenorhabditis elegans and Comparison to Spaceflight Experiments" Cells 12, no. 2: 270. https://doi.org/10.3390/cells12020270
ISSN2073-4409
URLhttps://udspace.udel.edu/handle/19716/32581
Languageen_US
PublisherCells
Keywordsceramide
Keywordssphinogolipid signaling
Keywordslongevity
Keywordstranscriptome
Keywordsspace
Keywordsintergenerational
Keywordsmicrogravity
TitleTranscriptomic Signature of the Simulated Microgravity Response in Caenorhabditis elegans and Comparison to Spaceflight Experiments
TypeArticle
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