Analyses of point mutation repair and allelic heterogeneity generated by CRISPR/Cas9 and single-stranded DNA oligonucleotides
| Author(s) | Bialk,Pawel | |
| Author(s) | Sansbury,Brett | |
| Author(s) | Rivera-Torres,Natalia | |
| Author(s) | Bloh,Kevin | |
| Author(s) | Man,Dula | |
| Author(s) | Kmiec,Eric B. | |
| Ordered Author | Pawel Bialk, Brett Sansbury, Natalia Rivera-Torres, Kevin Bloh, Dula Man,Eric B. Kmiec | |
| UD Author | Kmiec, Eric | |
| Date Accessioned | 2017-07-13T20:01:49Z | |
| Date Available | 2017-07-13T20:01:49Z | |
| Copyright Date | The Author(s) 2016 | |
| Publication Date | 9/9/16 | |
| Description | Publisher's PDF | |
| Abstract | The repair of a point mutation can be facilitated by combined activity of a single-stranded oligonucleotide and a CRISPR/Cas9 system. While the mechanism of action of combinatorial gene editing remains to be elucidated, the regulatory circuitry of nucleotide exchange executed by oligonucleotides alone has been largely defined. The presence of the appropriate CRISPR/Cas9 system leads to an enhancement in the frequency of gene editing directed by single-stranded DNA oligonucleotides. While CRISPR/Cas9 executes double-stranded DNA cleavage efficiently, closure of the broken chromosomes is dynamic, as varying degrees of heterogeneity of the cleavage products appear to accompany the emergence of the corrected base pair. We provide a detailed analysis of allelic variance at and surrounding the target site. In one particular case, we report sequence alteration directed by a distinct member of the same gene family. Our data suggests that single-stranded DNA molecules may influence DNA junction heterogeneity created by CRISPR/Cas9. | |
| Department | University of Delaware, College of Health Sciences, Department of Medical Laboratory Sciences | |
| Citation | Bialk, P., Sansbury, B., Rivera-Torres, N., Bloh, K., Man, D., & Kmiec, E. B. (2016). Analyses of point mutation repair and allelic heterogeneity generated by CRISPR/Cas9 and single-stranded DNA oligonucleotides. Scientific Reports, 6 doi:10.1038/srep32681 | |
| DOI | 10.1038/srep32681 | |
| ISSN | 2045-2322 | |
| URL | http://udspace.udel.edu/handle/19716/21536 | |
| Language | English | |
| Publisher | Nature Publishing Group | |
| dc.rights | CC BY 4.0 | |
| dc.source | Scientific Reports | |
| dc.source.uri | https://www.nature.com/articles/srep32681 | |
| Title | Analyses of point mutation repair and allelic heterogeneity generated by CRISPR/Cas9 and single-stranded DNA oligonucleotides | |
| Type | Article |
Files
Original bundle
1 - 1 of 1
Loading...
- Name:
- Analyses of point mutation repair and allelic heterogeneity generated by CRISPRCas9 and single-stranded DNA oligonucleotides.pdf
- Size:
- 1.43 MB
- Format:
- Adobe Portable Document Format
- Description:
- Main article