Atomic-resolution characterization of actin-binding protein, cofilin-2, in complex with two nucleotide-states of F-actin, by magic angle spinning NMR spectroscopy
| Author(s) | Yehl, Jenna B. | |
| Date Accessioned | 2017-06-20T11:26:33Z | |
| Date Available | 2017-06-20T11:26:33Z | |
| Publication Date | 2016 | |
| Abstract | Cofilin, an actin-binding protein, is critical for actin dynamics within the cell. Actin dynamics are responsible within the cell for many essential cellular processes such as cell motility, cell division and endocytosis. Cellular processes are related via actin-binding proteins and the nucleotide state of F-actin. Actin polymerization/depolymerization and filament severing have been extensively studied via X-ray fibre diffraction and cryo-electron microscopy; however, atomic-level structural information of actin associated proteins assembled on F-actin remained inaccessible. ☐ Here we report the first atomic-level resolution characterization of cofilin-2 assembled with F-actin in two nucleotide states (F-actin-ADP and F-actin-ADP-BeFx), by high-resolution magic-angle-spinning NMR. We have characterized the intermolecular interface of cofilin-2 on actin filaments in two nucleotide states. The results revealed remarkable spectral resolution enabling in-depth characterization of these systems. The MAS NMR approach presented here establishes the foundation for atomic-resolution structural analysis of a broad range of actin-associated proteins assembled on F-actin. | en_US |
| Advisor | Polenova, Tatyana | |
| Degree | Ph.D. | |
| Department | University of Delaware, Department of Chemistry and Biochemistry | |
| Unique Identifier | 990292302 | |
| URL | http://udspace.udel.edu/handle/19716/21472 | |
| Publisher | University of Delaware | en_US |
| URI | https://search.proquest.com/docview/1870036601?accountid=10457 | |
| dc.subject.lcsh | Actin. | |
| dc.subject.lcsh | Carrier proteins. | |
| dc.subject.lcsh | Nucleotides. | |
| dc.subject.lcsh | High resolution spectroscopy. | |
| dc.subject.lcsh | Nuclear magnetic resonance spectroscopy. | |
| Title | Atomic-resolution characterization of actin-binding protein, cofilin-2, in complex with two nucleotide-states of F-actin, by magic angle spinning NMR spectroscopy | en_US |
| Type | Thesis | en_US |