Identification of fibulin-1 as a human bone marrow stromal (HS-5) derived factor that induces prostate cancer cell death
| Author(s) | Nopmongkol, Kornkamon | |
| Date Accessioned | 2015-10-21T13:35:38Z | |
| Date Available | 2015-10-21T13:35:38Z | |
| Publication Date | 2015 | |
| Abstract | Previous studies showed that HS-5 cells secret unidentified factor(s) inducing PCa cell death. Herein, the HS-5 derived factor (HS-5 DF) was characterized and identified. HS-5 DF had protein characteristics being trypsin and heat sensitive, but pH stable. The tentative size of the factor fell between 30 kDa and 100 kDa. Disulfide bonding was important for HS-DF activity. TGF-?1 treatment led to a suppression of HS-5 DF activity, a property consistent with bone metastasis in prostate cancer. Examination of TGF-?1 down regulated proteins in HS-5 cell CM lead to identification of fibulin-1 as a candidate for the DF. Immunoprecipitation of fibulin-1 from HS-5 CM and CRISPR knockdown of Fibulin-1 showed a significant reduction of HS-5 CM derived PCa cell death. These results strongly support a role for fibulin-1 in HS-5 bone marrow stromal cell induction of PCa cell death. | en_US |
| Advisor | Sikes, Robert A. | |
| Degree | Ph.D. | |
| Department | University of Delaware, Department of Biological Sciences | |
| Unique Identifier | 925801700 | |
| URL | http://udspace.udel.edu/handle/19716/17183 | |
| Publisher | University of Delaware | en_US |
| URI | http://search.proquest.com/docview/1708646726?accountid=10457 | |
| dc.subject.lcsh | Mesenchymal stem cells. | |
| dc.subject.lcsh | Prostate -- Cancer. | |
| dc.subject.lcsh | Cancer cells. | |
| dc.subject.lcsh | Cell death. | |
| dc.subject.lcsh | Glycoproteins. | |
| dc.subject.lcsh | Calcium-binding proteins. | |
| dc.subject.lcsh | Transforming growth factors-beta. | |
| Title | Identification of fibulin-1 as a human bone marrow stromal (HS-5) derived factor that induces prostate cancer cell death | en_US |
| Type | Thesis | en_US |