A new approach for annotation of transposable elements using small RNA mapping

Author(s)El Baidouri, Moaine
Author(s)Do Kim, Kyung
Author(s)Abernathy, Brian
Author(s)Arikit, Siwaret
Author(s)Maumus, Florian
Author(s)Panaud, Olivier
Author(s)Meyers, Blake C.
Author(s)Jackson, Scott A.
Ordered AuthorMoaine El Baidouri, Kyung Do Kim, Brian Abernathy, Siwaret Arikit, Florian Maumus, Olivier Panaud, Blake C. Meyers and Scott A. Jackson
UD AuthorArikit, Siwareten_US
UD AuthorMeyers, Blake C. (orcid.org/0000-0003-3436-6097)en_US
Date Accessioned2015-11-30T19:01:50Z
Date Available2015-11-30T19:01:50Z
Copyright DateCopyright ©2015 The Authorsen_US
Publication Date2015-03-15
DescriptionPublisher's PDFen_US
AbstractTransposable elements (TEs) are mobile genomic DNA sequences found in most organisms. They so densely populate the genomes of many eukaryotic species that they are often the major constituents. With the rapid generation of many plant genome sequencing projects over the past few decades, there is an urgent need for improved TE annotation as a prerequisite for genome-wide studies. Analogous to the use of RNA-seq for gene annotation, we propose a new method for de novo TE annotation that uses as a guide 24 nt-siRNAs that are a part of TE silencing pathways. We use this new approach, called TASR (for Transposon Annotation using Small RNAs), for de novo annotation of TEs in Arabidopsis, rice and soybean and demonstrate that this strategy can be successfully applied for de novo TE annotation in plants.en_US
DepartmentDelaware Biotechnology Institute.en_US
DepartmentUniversity of Delaware. Department of Plant and Soil Sciences.en_US
CitationEl Baidouri, M., Do Kim, K., Abernathy, B., Arikit, S., Maumus, F., Panaud, O., ... & Jackson, S. A. (2015). A new approach for annotation of transposable elements using small RNA mapping. Nucleic acids research, gkv257.en_US
DOI10.1093/nar/gkv257en_US
ISSN0305-1048 ; e-1362-4962en_US
URLhttp://udspace.udel.edu/handle/19716/17246
Languageen_USen_US
PublisherOxford University Pressen_US
dc.rightsCC-BY 3.0 https://creativecommons.org/licenses/by/3.0/us/ Authors hold the copyright.en_US
dc.sourceNucleic Acids Researchen_US
dc.source.urihttp://nar.oxfordjournals.org/en_US
TitleA new approach for annotation of transposable elements using small RNA mappingen_US
TypeArticleen_US
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