Spatial transcriptomics reveals alterations in perivascular macrophage lipid metabolism in the onset of Wooden Breast myopathy in broiler chickens

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Author(s)Wang, Ziqing
Author(s)Khondowe, Paul
Author(s)Brannick, Erin
Author(s)Abasht, Behnam
Date Accessioned2024-04-25T18:28:07Z
Date Available2024-04-25T18:28:07Z
Publication Date2024-02-11
DescriptionThis article was originally published in Scientific Reports. The version of record is available at: https://doi.org/10.1038/s41598-024-53904-5. © The Author(s) 2024 This research was featured in UDaily on 05/07/2024 at: https://www.udel.edu/udaily/2024/may/poultry-wooden-breast-syndrome-human-health-behnam-abasht-erin-brannick/
AbstractThis study aims to use spatial transcriptomics to characterize the cell-type-specific expression profile associated with the microscopic features observed in Wooden Breast myopathy. 1 cm3 muscle sample was dissected from the cranial part of the right pectoralis major muscle from three randomly sampled broiler chickens at 23 days post-hatch and processed with Visium Spatial Gene Expression kits (10X Genomics), followed by high-resolution imaging and sequencing on the Illumina Nextseq 2000 system. WB classification was based on histopathologic features identified. Sequence reads were aligned to the chicken reference genome (Galgal6) and mapped to histological images. Unsupervised K-means clustering and Seurat integrative analysis differentiated histologic features and their specific gene expression pattern, including lipid laden macrophages (LLM), unaffected myofibers, myositis and vasculature. In particular, LLM exhibited reprogramming of lipid metabolism with up-regulated lipid transporters and genes in peroxisome proliferator-activated receptors pathway, possibly through P. Moreover, overexpression of fatty acid binding protein 5 could enhance fatty acid uptake in adjacent veins. In myositis regions, increased expression of cathepsins may play a role in muscle homeostasis and repair by mediating lysosomal activity and apoptosis. A better knowledge of different cell-type interactions at early stages of WB is essential in developing a comprehensive understanding.
SponsorThis research is supported by U.S. Department of Agriculture, Agriculture, and Food Research Initiative competitive grant number 2021-67015- 34543. We would like to thank Jeffery Caplan, Jean Ross, and staff from the University of Delaware Bio-imaging center; along with Bruce Kingham and Mark Shaw from University of Delaware Sequencing and Genotyping Center at the Delaware Biotechnology Institute for their expertise and assistance with sample processing, imaging and RNA sequencing. We also thank Dr. Carl Schmidt for his insightful input during manuscript preparation.
CitationWang, Z., Khondowe, P., Brannick, E. et al. Spatial transcriptomics reveals alterations in perivascular macrophage lipid metabolism in the onset of Wooden Breast myopathy in broiler chickens. Sci Rep 14, 3450 (2024). https://doi.org/10.1038/s41598-024-53904-5
ISSN2045-2322
URLhttps://udspace.udel.edu/handle/19716/34311
Languageen_US
PublisherScientific Reports
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
Keywordsagricultural genetics
Keywordsmetabolic disorders
TitleSpatial transcriptomics reveals alterations in perivascular macrophage lipid metabolism in the onset of Wooden Breast myopathy in broiler chickens
TypeArticle
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