Unique genetic responses revealed in RNAseq of the spleen of chickens stimulated with lipopolysaccharide and short-term heat

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Author(s)Van Goor, Angelica
Author(s)Ashwell, Chris M.
Author(s)Persia, Michael E.
Author(s)Rothschild, Max F.
Author(s)Schmidt, Carl J.
Author(s)Lamont, Susan J.
Ordered AuthorAngelica Van Goor, Chris M. Ashwell, Michael E. Persia, Max F. Rothschild, Carl J. Schmidt, Susan J. Lamont
UD AuthorSchmidt, Carl J.en_US
Date Accessioned2018-06-07T15:28:53Z
Date Available2018-06-07T15:28:53Z
Copyright DateCopyright © 2017 Van Goor et al.en_US
Publication Date2017-02-06
DescriptionPublisher's PDFen_US
AbstractClimate change and disease have large negative impacts on poultry production, but little is known about the interactions of responses to these stressors in chickens. Fayoumi (heat and disease resistant) and broiler (heat and disease susceptible) chicken lines were stimulated at 22 days of age, using a 2x2x2 factorial design including: breed (Fayoumi or broiler), inflammatory stimulus (lipopolysaccharide (LPS) or saline), and temperature (35ÊC or 25ÊC). Transcriptional changes in spleens were analyzed using RNA-sequencing on the Illumina HiSeq 2500. Thirty-two individual cDNA libraries were sequenced (four per treatment) and an average of 22 million reads were generated per library. Stimulation with LPS induced more differentially expressed genes (DEG, log2 fold change 2 and FDR 0.05) in the broiler (N = 283) than the Fayoumi (N = 85), whereas heat treatment resulted in fewer DEG in broiler (N = 22) compared to Fayoumi (N = 107). The double stimulus of LPS+heat induced the largest numbers of changes in gene expression, for which broiler had 567 DEG and Fayoumi had 1471 DEG of which 399 were shared between breeds. Further analysis of DEG revealed pathways impacted by these stressors such as Remodelling of Epithelial Adherens Junctions due to heat stress, Granulocyte Adhesion and Diapedesis due to LPS, and Hepatic Fibrosis/Hepatic Stellate Cell Activation due to LPS+heat. The genes and pathways identified provide deeper understanding of the response to the applied stressors and may serve as biomarkers for genetic selection for heat and disease tolerant chickens.en_US
DepartmentUniversity of Delaware. Department of Animal and Food Sciences.en_US
CitationVan Goor A, Ashwell CM, Persia ME, Rothschild MF, Schmidt CJ, Lamont SJ (2017) Unique genetic responses revealed in RNA-seq of the spleen of chickens stimulated with lipopolysaccharide and short-term heat. PLoS ONE 12(2): e0171414. doi:10.1371/journal. pone.0171414en_US
DOI10.1371/journal.pone.0171414en_US
ISSN1932-6203en_US
URLhttp://udspace.udel.edu/handle/19716/23554
Languageen_USen_US
PublisherPLoS (Public Library of Science)en_US
dc.rightsArticle is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.en_US
dc.rightsThis is an open access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.sourcePLOS Oneen_US
dc.source.urihttp://journals.plos.org/plosone/en_US
TitleUnique genetic responses revealed in RNAseq of the spleen of chickens stimulated with lipopolysaccharide and short-term heaten_US
TypeArticleen_US
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